Taxonomic revision of Ascidiacea (Tunicata) from the upper continental slope off north-western Australia

A collection of ascidians from 100–1000 m deep off the north-western coast of Australia has yielded 19 species, including one new, two possibly endemic to the north-western coast of Australia, four well-known Indo-west Pacific tropical species and 12 species known from the deeper waters of the western Pacific, of which 11 had not previously been recorded from off the western Australian coast or from other parts of the Indian Ocean. A third of these 12 species displays adaptations for a reduction in filter-feeding capacity, as do other known species from deeper slope and abyssal habitats. Nevertheless a connection with fauna of deep ocean basins of the Indian Ocean is not apparent at this stage. The full range of characters associated with species, genera and families are discussed, and those contributing to the identification of immature or mutilated specimens, as well as those relevant to an assessment of phylogenetic relationships, are emphasized.     

西瓜高效再生系统的研究

采用不同培养基对西瓜(Citrullus Vulgaris)的子叶、下胚轴和茎尖进行分化培养．结果表明：以MS为基本培养基，附加BA0．4mg/L处理时，3d苗龄的子叶的分化频率最高，可达95．5％，12d左右可见不定芽出现．西瓜对卡那霉素(Km)比较敏感，当培养基中添加20mg/L的Km即能完全抑制不定芽和不定根的分化．     

温室栽培西瓜单叶光合作用对主要生态因子的响应

在早春茬塑料日光温室条件下,以两个温室型品种为试材研究了西瓜单个叶片光合作用对主要生态因子的响应特性.结果表明:温室西瓜单叶的饱和光强和CO2饱和点均较大田条件下的低,两个品种间光补偿点和表观量子产额有较大差异,黄皮西瓜品种宝冠的光补偿点和表观量子产额明显高于花皮品种新金兰的,但两个品种间饱和光强差异不大,均在1200μmol/(m2·s)左右.两个品种CO2饱和点和补偿点差异不大,分别为1 050～1 250 μL/L和20～22 μL/L,但宝冠的羧化效率和RuBP再生速率(分别为0.1060和47.3)明显高于新金兰的(分别为0.0684和32.9);两个品种西瓜单叶光合作用的适宜温度范围略有差异,宝冠的光合适温(25～35℃)低于新金兰的(30～40℃).宝冠西瓜单叶光合作用的适宜大气湿度范围为50%～70%,低于日光温室环境的正常大气湿度,新金兰光合作用更适宜在低湿的空气环境中进行.     

3个籽瓜栽培品种的染色体核型分析

对３个籽瓜栽培品种进行染色体制片和分析研究，３个籽瓜品种染色体数目和核型为：黑瓜籽２ｎ＝２ｘ＝２２＝２０ｍ＋２ｓｍ，核型为１Ａ型；红瓜籽２ｎ＝２ｘ＝２２＝２２ｍ，核型为１Ａ型；白瓜籽２ｎ＝２ｘ＝２２＝２０ｍ＋２ｓｍ，核型为１Ａ型。     

西瓜NPR1抗病基因的遗传转化

比较了不同预培养时间、侵染时间、共培养时间等因素对农杆菌转化西瓜的影响。西瓜遗传转化的适宜条件:3 d苗龄的子叶,预培养1 d,农杆菌侵染10~15 m in,共培养3 d;不定芽与根的潮霉素(Hyg)筛选浓度分别为20 m g.L-1和6 m g.L-1。对20株西瓜转化苗进行DNA提取,PCR检测,其中5株呈阳性,初步证明目的基因已经整合到西瓜基因组中。